Detection and Comparison of RAPD & SSR Primers in Genetic Diversity of Ocimum sanctum
نویسندگان
چکیده
Aims: To Analysis of genetic variability in Tulsi (Ocimum sanctum) genotypes by using RAPD & SSR Markers.
 Place and Duration Study: Department Plant Biotechnology at K.K.Wagh College Agricultural Biotechnology, Nashik.
 Methodology: Ocimum tenuiflorum Linn., commonly known as Tulsi, is an aromatic plant with significant traditional medicinal value. assess the diversity relatedness six (Krishna, Ram, Lavangi, Pandharpuri, Daisil, Kapoori), molecular techniques were employed. The collected from Nagarjuna Medicinal Aromatic Park Dr. P.D.K.V. Akola. DNA isolation was performed alcohol fixation without liquid nitrogen, analyzed primers for characterization.
 Results: Genetic analysis Kapoori) markers. Five produced 15 bands, 11 bands showing polymorphism (73.3%) 4 monomorphism (26.7%). PIC value ranged 0.28 to 0.49 (average: 0.40). Four generated 9 8 1 band monomorphism. 0.24 0.57 0.39). Jaccard coefficient revealed moderate high similarity (0.40 0.73) (0.44 0.88) analyses. UPGMA dendrogram separated into two main clusters. Cluster included Krishna, Daisil while cluster 2 consisted Kapoori Tulsi. also formed clusters, 1, Pandharpuri dissimilarity forming 2.
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ژورنال
عنوان ژورنال: International Journal of Enviornment and Climate Change
سال: 2023
ISSN: ['2581-8627']
DOI: https://doi.org/10.9734/ijecc/2023/v13i82102